Pathogenesis and immune response in resistant and susceptible cultivars of grapevine (Vitis spp.) against Elsinoë ampelina infection.
Identifieur interne : 000034 ( Main/Exploration ); précédent : 000033; suivant : 000035Pathogenesis and immune response in resistant and susceptible cultivars of grapevine (Vitis spp.) against Elsinoë ampelina infection.
Auteurs : Rui Han [République populaire de Chine] ; Wuchen Yin [République populaire de Chine] ; Bilal Ahmad [République populaire de Chine] ; Peijia Gao [République populaire de Chine] ; Zhi Li [République populaire de Chine] ; Xiping Wang [République populaire de Chine]Source :
- Phytopathology [ 0031-949X ] ; 2020.
Abstract
Elsinoë ampelina is the main cause of grape anthracnose, and the majority of grapevine cultivars are susceptible to this fungus. Some Chinese wild grape cultivars are resistant, however. It is therefore apt to compare the pathogenesis and immune responses in susceptible and resistant cultivars of grapevine to explore the detailed molecular and biochemical mechanisms of resistance to this fungus. In this study, ultrastructural and histopathological observations were used to demonstrate the resistance responses to E. ampelina in the resistant Chinese wild cultivar Vitis quinquangularis clone 'Shang-24' and the susceptible cultivars Vitis davidii cv. 'Tangwei' and Vitis vinifera cv. 'Thompson Seedless'. Seventy-two hours post-inoculation (hpi) with E. ampelina, brown necrotic spots were clearly visible on the leaves of the susceptible cultivars 'Tangwei' and 'Thompson Seedless.' The infection was characterized by rapid colonization of the host cells by hyphae and massive spread of the pathogen in the intercellular spaces, ultimately leading to host cell collapse, cuticle dissolution, and extensive hyphal growth. In the resistant clone 'Shang-24', the conidia were lysed, a large quantity of electronically dense matter appeared, the hyphal growth was suppressed, and the host cells remained intact. In addition, six genes associated with disease resistance were differentially expressed in the susceptible and resistant cultivars. These disease-related genes were significantly up-regulated following infection with E. ampelina. This study illustrates the differences in infection and colonization of E. ampelina in resistant and susceptible grape leaves.
DOI: 10.1094/PHYTO-03-20-0079-R
PubMed: 33079021
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Some Chinese wild grape cultivars are resistant, however. It is therefore apt to compare the pathogenesis and immune responses in susceptible and resistant cultivars of grapevine to explore the detailed molecular and biochemical mechanisms of resistance to this fungus. In this study, ultrastructural and histopathological observations were used to demonstrate the resistance responses to E. ampelina in the resistant Chinese wild cultivar Vitis quinquangularis clone 'Shang-24' and the susceptible cultivars Vitis davidii cv. 'Tangwei' and Vitis vinifera cv. 'Thompson Seedless'. Seventy-two hours post-inoculation (hpi) with E. ampelina, brown necrotic spots were clearly visible on the leaves of the susceptible cultivars 'Tangwei' and 'Thompson Seedless.' The infection was characterized by rapid colonization of the host cells by hyphae and massive spread of the pathogen in the intercellular spaces, ultimately leading to host cell collapse, cuticle dissolution, and extensive hyphal growth. In the resistant clone 'Shang-24', the conidia were lysed, a large quantity of electronically dense matter appeared, the hyphal growth was suppressed, and the host cells remained intact. In addition, six genes associated with disease resistance were differentially expressed in the susceptible and resistant cultivars. These disease-related genes were significantly up-regulated following infection with E. ampelina. This study illustrates the differences in infection and colonization of E. ampelina in resistant and susceptible grape leaves.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33079021</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2020</Year><Month>Oct</Month><Day>20</Day></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Pathogenesis and immune response in resistant and susceptible cultivars of grapevine (Vitis spp.) against Elsinoë ampelina infection.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-03-20-0079-R</ELocationID><Abstract><AbstractText>Elsinoë ampelina is the main cause of grape anthracnose, and the majority of grapevine cultivars are susceptible to this fungus. Some Chinese wild grape cultivars are resistant, however. It is therefore apt to compare the pathogenesis and immune responses in susceptible and resistant cultivars of grapevine to explore the detailed molecular and biochemical mechanisms of resistance to this fungus. In this study, ultrastructural and histopathological observations were used to demonstrate the resistance responses to E. ampelina in the resistant Chinese wild cultivar Vitis quinquangularis clone 'Shang-24' and the susceptible cultivars Vitis davidii cv. 'Tangwei' and Vitis vinifera cv. 'Thompson Seedless'. Seventy-two hours post-inoculation (hpi) with E. ampelina, brown necrotic spots were clearly visible on the leaves of the susceptible cultivars 'Tangwei' and 'Thompson Seedless.' The infection was characterized by rapid colonization of the host cells by hyphae and massive spread of the pathogen in the intercellular spaces, ultimately leading to host cell collapse, cuticle dissolution, and extensive hyphal growth. In the resistant clone 'Shang-24', the conidia were lysed, a large quantity of electronically dense matter appeared, the hyphal growth was suppressed, and the host cells remained intact. In addition, six genes associated with disease resistance were differentially expressed in the susceptible and resistant cultivars. These disease-related genes were significantly up-regulated following infection with E. ampelina. This study illustrates the differences in infection and colonization of E. ampelina in resistant and susceptible grape leaves.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Rui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Northwest Agriculture and Forestry University, 12469, Yangling, Shaanxi, China; hanrui1@nwafu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Wuchen</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Northwest Agriculture and Forestry University, 12469, Yangling, Shaanxi, China; yinwuchen123@nwafu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahmad</LastName><ForeName>Bilal</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Northwest Agriculture and Forestry University, 12469, Horticulture, yangling, yangling, Shaanxi, China, 712100; bajwa1999@nwafu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Peijia</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Northwest Agriculture and Forestry University, 12469, Yangling, Shaanxi, China; gjiap@nwafu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Zhi</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Northwest Agriculture and Forestry University, 12469, College of Horticulture, Taicheng Road No.3 Yangling, Shaanxi province, China, Yangling, Shaanxi, China, 712100.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>United States; lizhi@nwsuaf.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiping</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Taicheng Road No.3Yangling, China, 712100; wangxiping@nwsuaf.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Analytical and theoretical plant pathology</Keyword><Keyword MajorTopicYN="N">Plant Stress and Abiotic Disorders</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>20</Day><Hour>12</Hour><Minute>11</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33079021</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-03-20-0079-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Han, Rui" sort="Han, Rui" uniqKey="Han R" first="Rui" last="Han">Rui Han</name></noRegion><name sortKey="Ahmad, Bilal" sort="Ahmad, Bilal" uniqKey="Ahmad B" first="Bilal" last="Ahmad">Bilal Ahmad</name><name sortKey="Gao, Peijia" sort="Gao, Peijia" uniqKey="Gao P" first="Peijia" last="Gao">Peijia Gao</name><name sortKey="Li, Zhi" sort="Li, Zhi" uniqKey="Li Z" first="Zhi" last="Li">Zhi Li</name><name sortKey="Wang, Xiping" sort="Wang, Xiping" uniqKey="Wang X" first="Xiping" last="Wang">Xiping Wang</name><name sortKey="Yin, Wuchen" sort="Yin, Wuchen" uniqKey="Yin W" first="Wuchen" last="Yin">Wuchen Yin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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